QIAamp MinElute Virus kit effectively extracts viral nucleic acids from cerebrospinal fluids and nasopharyngeal swabs

Recovery of Bordetella pertussis from nasopharyngeal swabs.

Comparison of Three Methods for Hepatitis B Virus Dna Extraction from Infected Serum Samples

The presence of hepatitis B virus (HBV) DNA in serum is a reliable marker of viral replication and infectivity, and PCR, one of the molecular biology techniques, is becoming the preferred method for its detection. Fifty hepatitis B surface antigen (HBsAg)-positive serum samples were collected and used for DNA extraction using three methods, including QIAamp MinElute Virus Spin Kit, DNP Kit and ...

Evaluation of a QIAamp DNA stool purification kit for Shiga-toxigenic Escherichia coli detection in bovine fecal swabs by PCR.

A commercial kit intended for Taq polymerase inhibitor removal was tested to detect Shiga-toxigenic Escherichia coli (STEC) by polymersase chain reaction (PCR) directly from cattle fecal samples. Forty-five samples were analysed for the presence of stx genes. Results were compared to those obtained by two other methods: amplification of DNA purified by a non-commercial procedure (heat lysis pro...

Fetal nucleic acids in maternal body fluids: an update.

Our laboratory continues to be actively involved in the development of new biomarkers for prenatal diagnosis using maternal blood and amniotic fluid. We have also developed a mouse model that demonstrates that cell-free fetal (cff) DNA is detectable in the pregnant maternal mouse. In human maternal plasma and serum we have analyzed factors that are important in the clinical interpretation of cf...

Viral nucleic acids in live-attenuated vaccines: detection of minority variants and an adventitious virus.

Metagenomics and a panmicrobial microarray were used to examine eight live-attenuated viral vaccines. Viral nucleic acids in trivalent oral poliovirus (OPV), rubella, measles, yellow fever, varicella-zoster, multivalent measles/mumps/rubella, and two rotavirus live vaccines were partially purified, randomly amplified, and pyrosequenced. Over half a million sequence reads were generated covering...